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Team:Paris/Modeling/f1
From 2008.igem.org
(Difference between revisions)
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|- | |- | ||
|(γ K<sub>tet</sub> / {coef<sub>tetR</sub>} ƒ0) | |(γ K<sub>tet</sub> / {coef<sub>tetR</sub>} ƒ0) | ||
| - | |activation constant of pTet <br> K<sub> | + | |activation constant of pTet <br> K<sub>20</sub> |
|nM | |nM | ||
| | | | ||
|- | |- | ||
|n<sub>tet</sub> | |n<sub>tet</sub> | ||
| - | |complexation order of pTet<br> n<sub> | + | |complexation order of pTet<br> n<sub>20</sub> |
|no dimension | |no dimension | ||
| | | | ||
|- | |- | ||
|K<sub>aTc</sub> | |K<sub>aTc</sub> | ||
| - | |complexation constant aTc-TetR <br> K<sub> | + | |complexation constant aTc-TetR <br> K<sub>19</sub> |
|nM | |nM | ||
| | | | ||
|- | |- | ||
|n<sub>aTc</sub> | |n<sub>aTc</sub> | ||
| - | |complexation order aTc-TetR <br> n<sub> | + | |complexation order aTc-TetR <br> n<sub>19</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 10:03, 8 October 2008
The experience would give us
Thus, at steady-state and in the exponential phase of growth :
| param | signification | unit | value |
| [expr(pTet)] | expression rate of pTet with RBS E0032 | nM.min-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βtet | production rate of pTet with RBS E0032 β1 | nM.min-1 | |
| (γ Ktet / {coeftetR} ƒ0) | activation constant of pTet K20 | nM | |
| ntet | complexation order of pTet n20 | no dimension | |
| KaTc | complexation constant aTc-TetR K19 | nM | |
| naTc | complexation order aTc-TetR n19 | no dimension |
Also, this experiment will allow us to know the expression of ƒ1 :
