Team:Warsaw/Calendar-Main/16 September 2008
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
- | <p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p> | + | <p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li> |
+ | Tests for ampicillin resistance given by protein added to the medium: | ||
- | + | Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega | |
- | |||
- | + | Rivalry Tests: | |
- | + | Inoculation to one tube A-omega, omega and Z-omega - A_alpha protein added ~100 μg | |
- | + | (0,25IPTG; 50AP) A-omega, omega and Z-omega - Z_alfa protein added ~100 μg | |
- | + | A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg | |
- | (0,25IPTG; 50AP) | + | |
- | + | ||
Revision as of 16:42, 8 October 2008