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Team:Warsaw/Calendar-Main/23 June 2008
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<h4>Michał L., Ewa, Marcin:</h4> | <h4>Michał L., Ewa, Marcin:</h4> | ||
<ol><li>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 12 μM.</li></ol> | <ol><li>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 12 μM.</li></ol> | ||
| - | + | <h3>Preparation of constructs with OmpA protein fusions</h3> | |
| + | <h4>Michał K.:</h4> | ||
| + | <ol> | ||
| + | <li> Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</li> | ||
| + | <li> | ||
| + | <li> PCR on isolated plasmids with </ol> | ||
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Revision as of 22:16, 8 October 2008
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PCR resultsMichał L., Ewa, Marcin:
Preparation of constructs with OmpA protein fusionsMichał K.:
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