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Team:Chiba/Project
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Quorum sensing bacteria have both signal sending and receiving abilities.We engineered two classes of E. coli which have one or the other of these abilities. | Quorum sensing bacteria have both signal sending and receiving abilities.We engineered two classes of E. coli which have one or the other of these abilities. | ||
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| + | ===Quorum-Sensing Cross-talk=== | ||
=== The Experiments === | === The Experiments === | ||
Revision as of 10:42, 9 October 2008
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Contents |
Project overview
E.coli time manager
- We control the timing of gene expression by using multiple signaling devices.To this end,we utilize molecules associated with Quorum sensing, a phenomenon that allows bacteria to communicate with each other.Our project uses two classes of bacteria: senders and receivers. Senders produce signaling molecules, and Receivers are activated only after a particular concentration of this molecule is reached.Although different quorum sensing species have slightly different signaling molecules, these molecules are not completely specific to their hosts and cross-species reactivity is observed.Communication using non-endogenous molecules is less sensitive, andrequires a higher signal concentration to take effect.This results in slower activation of receivers.
Project Details
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Quorum sensing bacteria have both signal sending and receiving abilities.We engineered two classes of E. coli which have one or the other of these abilities.
Quorum-Sensing Cross-talk
The Experiments
Part 3
Results
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