Team:Paris/Modeling/f1

From 2008.igem.org

(Difference between revisions)
Line 21: Line 21:
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
|
|
-
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
+
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures with well choosen [aTc]<sub>i</sub>
|-
|-
|γ<sub>GFP</sub>
|γ<sub>GFP</sub>

Revision as of 13:15, 9 October 2008

F1a.jpg

The experience would give us

F1expr.jpg

Thus, at steady-state and in the exponential phase of growth :

ExprptetF0.jpg


param signification unit value comments
[expr(pTet)] expression rate of
pTet with RBS E0032
nM.min-1 see "findparam"
need for 20 measures with well choosen [aTc]i
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198 Time for Cell Division : 35 min. ; No degradation
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
βtet production rate of pTet with RBS E0032
β1
nM.min-1
(Ktet/{coeftetR}) activation constant of pTet
K20
nM The optimisation program will give us (γ Ktet / {coeftetR} ƒ0) The litterature [?] gives Ktet =
ntet complexation order of pTet
n20
no dimension The litterature [?] gives ntet =
KaTc complexation constant aTc-TetR
K19
nM The litterature [?] gives KaTc =
naTc complexation order aTc-TetR
n19
no dimension The litterature [?] gives naTc =

Also, this experiment will allow us to know the expression of ƒ1 :

ExprF1.jpg