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Team:Paris/Modeling/f2
From 2008.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
|unit | |unit | ||
|value | |value | ||
| + | |comments | ||
|- | |- | ||
|[expr(pBad)] | |[expr(pBad)] | ||
|expression rate of <br> pBad '''with RBS E0032''' | |expression rate of <br> pBad '''with RBS E0032''' | ||
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| - | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures | + | | |
| + | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures with well choosen [arab]<sub>i</sub> | ||
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
| Line 28: | Line 30: | ||
|GFP concentration at steady-state | |GFP concentration at steady-state | ||
|nM | |nM | ||
| + | | | ||
|need for 20 measures | |need for 20 measures | ||
|- | |- | ||
| Line 33: | Line 36: | ||
|value of the observed fluorescence | |value of the observed fluorescence | ||
|au | |au | ||
| + | | | ||
|need for 20 measures | |need for 20 measures | ||
|- | |- | ||
| Line 39: | Line 43: | ||
|nM.au<sup>-1</sup> | |nM.au<sup>-1</sup> | ||
|(1/79.429) | |(1/79.429) | ||
| + | | | ||
|} | |} | ||
| Line 48: | Line 53: | ||
|unit | |unit | ||
|value | |value | ||
| + | |comments | ||
|- | |- | ||
|β<sub>bad</sub> | |β<sub>bad</sub> | ||
|production rate of pBad '''with RBS E0032''' <br> not in the system | |production rate of pBad '''with RBS E0032''' <br> not in the system | ||
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| + | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>bad</sub>/ | + | |(γ K<sub>bad</sub>/''const.expr(pBad)'') |
|activation constant of pBad <br> not in the system | |activation constant of pBad <br> not in the system | ||
|nM | |nM | ||
| + | | | ||
| | | | ||
|- | |- | ||
| Line 62: | Line 70: | ||
|complexation order of pBad<br> not in the system | |complexation order of pBad<br> not in the system | ||
|no dimension | |no dimension | ||
| + | | | ||
| | | | ||
|- | |- | ||
| Line 67: | Line 76: | ||
|complexation constant Arabinose-AraC <br> not in the system | |complexation constant Arabinose-AraC <br> not in the system | ||
|nM | |nM | ||
| + | | | ||
| | | | ||
|- | |- | ||
| Line 72: | Line 82: | ||
|complexation order Arabinose-AraC <br> not in the system | |complexation order Arabinose-AraC <br> not in the system | ||
|no dimension | |no dimension | ||
| + | | | ||
| | | | ||
|} | |} | ||
That will give us directly ƒ2([arab]) | That will give us directly ƒ2([arab]) | ||
Revision as of 13:28, 9 October 2008
The experience would give us
Thus, at steady-state and in the exponential phase of growth :
| param | signification | unit | value | comments |
| [expr(pBad)] | expression rate of pBad with RBS E0032 | nM.min-1 | see "findparam" need for 20 measures with well choosen [arab]i | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| βbad | production rate of pBad with RBS E0032 not in the system | nM.min-1 | ||
| (γ Kbad/const.expr(pBad)) | activation constant of pBad not in the system | nM | ||
| nbad | complexation order of pBad not in the system | no dimension | ||
| Kara | complexation constant Arabinose-AraC not in the system | nM | ||
| nara | complexation order Arabinose-AraC not in the system | no dimension |
That will give us directly ƒ2([arab])
