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Team:Warsaw/Calendar-Main/29 July 2008
From 2008.igem.org
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Checking if OmpA_omega_A_alpha gives ampicillin resistance.<br> | Checking if OmpA_omega_A_alpha gives ampicillin resistance.<br> | ||
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Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 (repeat is needed!!!)<br> | Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 (repeat is needed!!!)<br> | ||
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Checking OmpA_omega_A_alpha and OmpA_A_alpha expression.<br> | Checking OmpA_omega_A_alpha and OmpA_A_alpha expression.<br> | ||
Revision as of 14:11, 9 October 2008
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1. Separate transformant colonies (transformation from previous day) inoculated to liquid LB with kanamycin. Piotr:
Checking if OmpA_omega_A_alpha gives ampicillin resistance.Inoculation OmpA_omega_A_alpha from various IPTG concentrations (0, 0.1, 0.25, 0.5, 0.75, 1) into the same IPTG concentrations, but with various ampicillin concentrations(25, 50, 75, 100) in relation: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 (repeat is needed!!!) Checking OmpA_omega_A_alpha and OmpA_A_alpha expression.
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