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Team:Warsaw/Calendar-Main/13 May 2008
From 2008.igem.org
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20 cycles </li> | 20 cycles </li> | ||
| - | <img src="https://static.igem.org/mediawiki/2008/7/71/Aid_pcr_grad_WAW.jpg" width=350/><var>1-DNA ladder; 2 to 13-PCR products | + | <img src="https://static.igem.org/mediawiki/2008/7/71/Aid_pcr_grad_WAW.jpg" width=350/><var>Gradient PCR products: 1-DNA ladder; 2 to 13-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 13)</var> |
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<li> Optimization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C). <br> | <li> Optimization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C). <br> | ||
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20 cycles<br> | 20 cycles<br> | ||
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| + | <img src="https://static.igem.org/mediawiki/2008/6/6c/T7grad_WAW2.jpg" width=350/> | ||
| + | <var>Gradient PCR products for translation fusion (only negative control-Top10 genomic DNA): 1-DNA ladder; 2 to 6-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 6).</var> | ||
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</li> | </li> | ||
<li> Optimization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for transcription fusion; annealing temperature gradient (from 62°C to 82°C). <br> | <li> Optimization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for transcription fusion; annealing temperature gradient (from 62°C to 82°C). <br> | ||
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20 cycles <br> | 20 cycles <br> | ||
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| + | <img src="https://static.igem.org/mediawiki/2008/9/97/T7grad_WAW1.jpg" width=350/> | ||
| + | <var>Gradient PCR products: upper - for transcription fusion, lower - for translation fusion; 1-DNA ladder; 2 to 12-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 12)</var> | ||
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</li> | </li> | ||
<li> Gel electrophoresis of PCR products (there wasn't any products for T7 RNA polymerase for translation fusion).</li> | <li> Gel electrophoresis of PCR products (there wasn't any products for T7 RNA polymerase for translation fusion).</li> | ||
</ol> | </ol> | ||
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</p> | </p> | ||
Revision as of 14:22, 9 October 2008
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Preparation of pMPMT5+AID construct and PCRs for fusions
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Gradient PCR products: 1-DNA ladder; 2 to 13-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 13)
Gradient PCR products for translation fusion (only negative control-Top10 genomic DNA): 1-DNA ladder; 2 to 6-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 6).
Gradient PCR products: upper - for transcription fusion, lower - for translation fusion; 1-DNA ladder; 2 to 12-PCR products-border annealing temperatures of gradient: 62°C (lane 2) to 82°C (lane 12)
