Team:Warsaw/Calendar-Main/22 May 2008

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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
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<h4>Piotr:</h4>
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<h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7<br>
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Piotr</h3>
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Design of primers for sequencing something we want from pMPM-T5.</li><br>
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Design of primers for sequencing something we want from pMPM-T5.<br>
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<p>Primers:<br>
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Primers:<br>
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a></li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a></li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
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<h4>Michał K.:</h4>
<h4>Michał K.:</h4>
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<li> Gel electrophoresis (no proper colonies found).</li>
<li> Gel electrophoresis (no proper colonies found).</li>
<img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/>
<img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/>
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<h3>1-DNA ladder;<br>  
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<var>1-DNA ladder;<br>  
2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); <br>
2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); <br>
4-pMPM-T5-AID  digested with EcoRI; <br>
4-pMPM-T5-AID  digested with EcoRI; <br>
5-hypothetical pMPM with translation fusion digested with BamHI; <br>
5-hypothetical pMPM with translation fusion digested with BamHI; <br>
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6-pMPM-T5-AID digested with BamHI.</h3>
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6-pMPM-T5-AID digested with BamHI.</var>
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Revision as of 14:37, 9 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7
Piotr

Design of primers for sequencing something we want from pMPM-T5.
Primers:

Michał K.:

  1. Isolation of hypothetical pMPM-T5 with transcription fusion.
  2. Isolation of hypothetical pMPM-T5 with translation fusion.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translation fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (no proper colonies found).
    6. 1-DNA ladder;
    2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones);
    4-pMPM-T5-AID digested with EcoRI;
    5-hypothetical pMPM with translation fusion digested with BamHI;
    6-pMPM-T5-AID digested with BamHI.

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