Team:Warsaw/Calendar-Main/25 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a></li>
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<li> Lack of confirmed transformant colonies </li></ol></p>
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Revision as of 15:10, 9 October 2008

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Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
  2. Lack of confirmed transformant colonies


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