Team:Warsaw/Calendar-Main/28 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
 
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin
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Antoni</h3>
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<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa.</li></ol></p>
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Revision as of 15:12, 9 October 2008

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Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Isolation of pET15b+OmpA-alfa.

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