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Team:Paris/Modeling/f2
From 2008.igem.org
(Difference between revisions)
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|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
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| - | | | + | |need for 20 measures with well choosen [arab]<sub>i</sub> |
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|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
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|no dimension | |no dimension | ||
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| - | | | + | |The literature [[Team:Paris/Modeling/Bibliography|[?]]] gives n<sub>bad</sub> = |
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|K<sub>ara</sub> | |K<sub>ara</sub> | ||
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|nM | |nM | ||
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| - | | | + | |The literature [[Team:Paris/Modeling/Bibliography|[?]]] gives K<sub>ara</sub> = |
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|n<sub>ara</sub> | |n<sub>ara</sub> | ||
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|no dimension | |no dimension | ||
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| - | | | + | |The literature [[Team:Paris/Modeling/Bibliography|[?]]] gives n<sub>ara</sub> = |
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That will give us directly ƒ2([arab]) | That will give us directly ƒ2([arab]) | ||
Revision as of 15:23, 9 October 2008
The experience would give us
Thus, at steady-state and in the exponential phase of growth :
| param | signification | unit | value | comments |
| [expr(pBad)] | expression rate of pBad with RBS E0032 | nM.min-1 | need for 20 measures with well choosen [arab]i | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| βbad | production rate of pBad with RBS E0032 not in the system | nM.min-1 | ||
| (γ Kbad/const.expr(pBad)) | activation constant of pBad not in the system | nM | ||
| nbad | complexation order of pBad not in the system | no dimension | The literature [?] gives nbad = | |
| Kara | complexation constant Arabinose-AraC not in the system | nM | The literature [?] gives Kara = | |
| nara | complexation order Arabinose-AraC not in the system | no dimension | The literature [?] gives nara = |
That will give us directly ƒ2([arab])
