Team:Warsaw/Calendar-Main/24 June 2008

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<h3>PCR results <br>Michał K. </h3>
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Michał L., Ewa, Marcin</h3>
Still no success. We need to run gradient PCR to find optimal reaction conditions.  
Still no success. We need to run gradient PCR to find optimal reaction conditions.  

Revision as of 20:26, 9 October 2008

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PCR results
Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Preparation of constructs with OmpA protein fusions
Michał K.

  1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers
  2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles)

We need a title here

Sequencing determined that all white colonies have intact lacZ gene.

Reason of white phenotype unknown.

We suppose that it is due to chromosomal mutation.

We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).

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