From 2008.igem.org
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| - | <h3>Optimisation of primers from 19.06</h3> | + | <h3>Paweł</h3> |
| - | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pDRIVE-TapTag plasmid using | + | <p><ol><li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/ igem_notebook.htm# digest"> digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols# removing"> dephosphorylation with CIAP</a>.</li></ ol></p> |
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
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| - | primers (annealing length 45s)</li>
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols# pcr"> PCR</a> on pUC19 plasmid using | + | |
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
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| - | primers (annealing length 45s)</li>
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| - | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pUC19 plasmid using | + | |
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
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| - | primers (annealing length 30s)</li>
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| - | <p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p>
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Revision as of 21:08, 9 October 2008
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