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Team:Warsaw/Calendar-Main/2 July 2008
From 2008.igem.org
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| - | + | <h3>Preparation of constructs with OmpA protein fusions | |
| - | <h3>Preparation of constructs with OmpA protein fusions<br> | + | <br>Paweł</h3> |
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| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest.</li> | ||
| + | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b. </li> | ||
| + | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b.</li> | ||
| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a> strain with ligation products. </li> | ||
| + | <li> Transformants plating on LB + ampicillin.</li></ol> | ||
| + | </p> | ||
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Revision as of 21:30, 9 October 2008
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Preparation of constructs with OmpA protein fusions
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