Team:Warsaw/Calendar-Main/4 July 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
 +
<html>
 +
<h3>Preparation of constructs with OmpA protein fusions<br>Paweł</h3>
 +
<p><ol>
 +
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega). </li>
 +
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with sacI and BamHI. </li>
 +
<li> Gel electrophoresis. </li>
 +
</ol></p>
 +
 +
 +
</html>

Revision as of 21:43, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Preparation of constructs with OmpA protein fusions
Paweł

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega).
  2. Control digest of plasmids with sacI and BamHI.
  3. Gel electrophoresis.