"
Team:UNIPV-Pavia/Protocols/GelExtraction
From 2008.igem.org
(Difference between revisions)
| Line 34: | Line 34: | ||
'''Materials needed:''' | '''Materials needed:''' | ||
*'''A gel in which the bands of interest can be seen''' | *'''A gel in which the bands of interest can be seen''' | ||
| + | *'''Agarose gel with well-separated, sharp bands''' | ||
*'''Scalpel and tweezers to cut gel''' | *'''Scalpel and tweezers to cut gel''' | ||
*'''Roche Agarose Gel DNA Extraction Kit''' | *'''Roche Agarose Gel DNA Extraction Kit''' | ||
Revision as of 09:21, 11 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
DNA gel extraction
(Estimated time: 1 hour)
Materials needed:
- A gel in which the bands of interest can be seen
- Agarose gel with well-separated, sharp bands
- Scalpel and tweezers to cut gel
- Roche Agarose Gel DNA Extraction Kit
- Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol
