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Team:UNIPV-Pavia/Protocols/GelExtraction
From 2008.igem.org
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'''Materials needed:''' | '''Materials needed:''' | ||
*'''Agarose gel with well-separated, sharp bands''' | *'''Agarose gel with well-separated, sharp bands''' | ||
| - | *'''Scalpel and tweezers to cut gel''' | + | *'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut) |
*'''Roche Agarose Gel DNA Extraction Kit''' | *'''Roche Agarose Gel DNA Extraction Kit''' | ||
<br> | <br> | ||
*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol] | *Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol] | ||
<br> | <br> | ||
Revision as of 09:22, 11 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
DNA gel extraction
(Estimated time: 1 hour)
Materials needed:
- Agarose gel with well-separated, sharp bands
- Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
- Roche Agarose Gel DNA Extraction Kit
- Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol
