Team:Warsaw/Calendar-Main/27 June 2008

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<h3>Preparation of constructs with OmpA protein fusions<br>Michał K.</h3>
<h3>Preparation of constructs with OmpA protein fusions<br>Michał K.</h3>
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products. </li></ol>
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products. </li></ol>
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<h3>Cloning PLC products on pKS<h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<ol>
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<li>Gel electophoresis of PCL products</li>
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<li>Gel-out of proper bands</li>
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<li>Restriction digest of pKS vector and the PCL products with NotI and SacI for 2 hours</li>
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<li>Ligation 1 hour</li>
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<li>Transformation of Top10 strain and screening on Amp<sub>100</sub> plates</li>
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</ol>

Revision as of 13:03, 10 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Clean-up of OmpA_alpha and OmpA_omega digest products.

Cloning PLC products on pKS

Michał L., Ewa, Marcin:

  1. Gel electophoresis of PCL products
  2. Gel-out of proper bands
  3. Restriction digest of pKS vector and the PCL products with NotI and SacI for 2 hours
  4. Ligation 1 hour
  5. Transformation of Top10 strain and screening on Amp100 plates

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