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Team:Warsaw/Calendar-Main/3 July 2008
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Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.</li></ol> | Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.</li></ol> | ||
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| + | <h3>Cloning alpha-A and omega-A fusions on pKS</h3> | ||
| + | <h4>Michał L., Ewa, Marcin:</h4> | ||
| + | <p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p> | ||
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Revision as of 13:37, 10 October 2008
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Preparation of constructs with OmpA protein fusions
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