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Team:UNIPV-Pavia/Protocols/Digestion
From 2008.igem.org
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'''Materials needed:''' | '''Materials needed:''' | ||
| - | *'''Roche''' | + | *'''Roche restriction enzymes thawed on ice''' |
*'''Pre-warmed at 37°C bath''' | *'''Pre-warmed at 37°C bath''' | ||
*'''Cut and gel-extracted vector''' | *'''Cut and gel-extracted vector''' | ||
Revision as of 12:24, 11 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
BioBrick digestion with restriction enzymes
(estimated time: 2 hours and 15 min)
Materials needed:
- Roche restriction enzymes thawed on ice
- Pre-warmed at 37°C bath
- Cut and gel-extracted vector
