Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

1. Isolation of plasmids from cultures inocluated on previous day
2. Control digest of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors
3. Another overnight ligation of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA

Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

1. Since the phage is gone we can continue this cloning:
2. Restriction digest of PCL product and pKS vector with SacI and NotI
3. Ligation of omega-A fusion with pKS vector
4. Transformation of Top10 with ligation product