Team:Warsaw/Calendar-Main/22 July 2008

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<h3>Cloning omega-A fusion on pKS (second attempt)</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<li>Isolation of plasmids from transformants</li>
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<li>Digest of plasmids with SacI and NotI</li>
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<li>Gel electrophoresis of products</li>
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<li>2 selected clones were send to DNA sequencing lab</li>
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Revision as of 14:24, 10 October 2008

Gallery Bricks Notebook Team Project Home


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Cloning of protein A DNA to OmpA constructs

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
  2. Control digest of isolated plasmids with BamHI and SacI.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin

Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

  1. Isolation of plasmids from transformants
  2. Digest of plasmids with SacI and NotI
  3. Gel electrophoresis of products
  4. 2 selected clones were send to DNA sequencing lab

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