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Team:Hawaii/Notebook/2008-10-10
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===verification of Transformants=== :<strong> Grace</strong> [[Image:101008colonypcr.jpg|right|thumb|500px|EtBr stained 2%...) |
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= Things we did today = | = Things we did today = | ||
== Wetlab work == | == Wetlab work == | ||
| - | === | + | ===Verification of Transformants=== |
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
[[Image:101008colonypcr.jpg|right|thumb|500px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.]] | [[Image:101008colonypcr.jpg|right|thumb|500px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.]] | ||
| Line 30: | Line 30: | ||
:* Colony PCR of transformants | :* Colony PCR of transformants | ||
| + | ===Overnight RE digest=== | ||
| + | :<strong>Grace</strong> | ||
| + | :* EcoRI/PstI: | ||
| + | ::* BBpRL1383a-1 | ||
| + | ::* nrsg #6 (PCR) | ||
| + | ::* prpgt #5, 7, 11 (PCR) | ||
===Triparental Conjugation=== | ===Triparental Conjugation=== | ||
Revision as of 02:31, 11 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of Transformants
- Grace
File:101008colonypcr.jpg
EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.
| Construct | Colonies |
|---|---|
| nir+rbs+GFP+tt#1 | 100 |
| plac+rbs+GFP+tt#1 | 3 |
| nir+rbs+slr1+GFPf | 51 |
| nir+rbs+pilA+GFPf+tt | 24 |
| BBpRL1383a | lawn |
| (-) no DNA | 0 |
- Colony PCR of transformants
Overnight RE digest
- Grace
- EcoRI/PstI:
- BBpRL1383a-1
- nrsg #6 (PCR)
- prpgt #5, 7, 11 (PCR)
Triparental Conjugation
- Grace
- Began triparental conjugation between E. coli containing RP1 and BBpRL1383a -1 or -18 and Synechocystis
- RP1 OD600=0.6341
- BBpRL1383-a OD600=61.25
- BBpRL1383a-18 OD600=0.4884
- Synechocystis OD700=0.8629, OD730=0.7337
- Placed plates in SC 37C CO2 incubator until Monday
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
