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Team:Warsaw/Calendar-Main/6 May 2008
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<h4>Piotr:</h4> | <h4>Piotr:</h4> | ||
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| - | + | <li>Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">electro-</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.</li> | |
| - | + | <li>Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.</li></ol><br> | |
| - | < | + | <h3> Isolation of genomic DNA </h3> |
<h4>Michał L.:</h4> | <h4>Michał L.:</h4> | ||
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| - | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from <i>E. coli</i> TOP10 strain was used as a negative control.<br> | |
</p> | </p> | ||
Revision as of 08:37, 11 October 2008
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Piotr:
Isolation of genomic DNAMichał L.:
Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control. 
Probes of isolated genomic DNA after mechanical destruction. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.
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