Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

(Difference between revisions)
Line 24: Line 24:
<h3> Blue/white and rifampicin test </h3>
<h3> Blue/white and rifampicin test </h3>
-
<h4>Michał L., Ewa, Marcin:</h4>  
+
<h4>Michał L., Ewa, Marcin</h4>  
<p>
<p>
<ol>
<ol>

Revision as of 09:36, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase.
Primers: AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20 and 25

Blue/white and rifampicin test

Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin (30 μg/mL) + X-gal + IPTG.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008"