Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

(Difference between revisions)
Line 13: Line 13:
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br>
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Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>
+
Template DNA: translation fusion AID+T7 RNA-polymerase (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>)<br>
Elongation temperature: 55°C<br>
Elongation temperature: 55°C<br>

Revision as of 10:02, 11 October 2008

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Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase.
Primers: AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20 and 25

Blue/white and rifampicin test

Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin (30 μg/mL) + X-gal + IPTG.

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