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Team:Warsaw/Calendar-Main/5 June 2008
From 2008.igem.org
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Inoculation of of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> into liquid LB broth (with 30 μg/ml of ampicillin).</p><br> | Inoculation of of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> into liquid LB broth (with 30 μg/ml of ampicillin).</p><br> | ||
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| - | <p>Primers: | + | <h4>Piotr</h4> |
| + | <p>Design of primers for sequencing β-galactosidase from <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>. <br> | ||
| + | Primers: | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and | ||
Revision as of 10:26, 11 October 2008
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Preparation of pMPMT5+T7 constructPiotr
Preparation of pMPMT5-AID+AIDT7 constructMichał K.Inoculation of colonies of transformants (pMPMT5-AID with AID-T7 RNA-polymerase translation fusion) into liquid LB+tetracycline. Blue/white and rifampicin testMichał L., Ewa, MarcinInoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin). PiotrDesign of primers for sequencing β-galactosidase from pZC320.
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