Team:Warsaw/Calendar-Main/26 June 2008

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<h3><h3>Preparation of constructs with OmpA protein fusions <br>Michał K.</h3>
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<h3>Preparation of constructs with OmpA protein fusions </h3><h4>Michał K.</h4>
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<li> Repetition of PCRs, gel-out isolations and digests from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008">17 June.</a></li></ol>
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<p>Repetition of PCRs, gel-out isolations and digests from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008">17 June.</a></p>
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<h3> Polymerase Chain Ligation (PCL) <br>Michał L., Ewa, Marcin</h3>
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<h3> Polymerase Chain Ligation (PCL) </h3><h4>Michał L., Ewa, Marcin</h4>
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Revision as of 11:32, 11 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs, gel-out isolations and digests from 17 June.

Polymerase Chain Ligation (PCL)

Michał L., Ewa, Marcin

ProductTemplatesPrimersProduct length
Alpha-A fusionAlpha-linker + linker-AAlphaL+SacI + AP+NotI 1500 bp
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp

PCL program for fusion A with alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-62°C0:45
68°C2:30
68°C10:00
4°Cinfinite

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