Team:Warsaw/Calendar-Main/4 June 2008
From 2008.igem.org
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of reaction products.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of reaction products.</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pMPM-T5 + AID with AID-T7 RNA-polymerase translation fusion).</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pMPM-T5 + AID with AID-T7 RNA-polymerase translation fusion).</li> | ||
- | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols# | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li> |
<li>Plating transformants on LB+tetracycline.</li></ol></html><br> | <li>Plating transformants on LB+tetracycline.</li></ol></html><br> | ||
<h3>Preparation of pMPMT5+T7 construct</h3> | <h3>Preparation of pMPMT5+T7 construct</h3> |
Revision as of 11:45, 11 October 2008
Preparation of pMPMT5-AID+AIDT7 constructMichał K.
Preparation of pMPMT5+T7 constructPiotrInoculation some separate transformant colonies (liquid LB + tetracycline).
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