Team:Warsaw/Calendar-Main/6 July 2008

From 2008.igem.org

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<tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr>
<tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr>
</table>
</table>
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+
<br>
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<h4>PCL program for omega-A fusion</h4>
+
<table id="result">
<table id="result">
 +
<tr><th colspan="3"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">PCL</a> program for omega-A fusion</th></tr>
<tr><th>Temperature</th><th>Time</th></tr>
<tr><th>Temperature</th><th>Time</th></tr>
<tr><td>94&deg;C</td><td>4:00</td></tr>
<tr><td>94&deg;C</td><td>4:00</td></tr>

Revision as of 12:43, 11 October 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

  1. Gel electrophoresis of PCR products
  2. Gel-out of proper bands
  3. PCL reaction to create omega-A fusion:

    ProductTemplatesPrimersProduct length
    Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp

    PCL program for omega-A fusion
    TemperatureTime
    94°C4:00
    94°C0:3028 cycles
    48°C-58°C0:45
    68°C2:00
    68°C10:00
    4°Cinfinite

  4. Gel electrophoresis of PCL products
  5. We have obtained no PCL product (weird?)