Team:Warsaw/Calendar-Main/2 July 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
-
<h3>Preparation of constructs with OmpA protein fusions
+
<h3>Preparation of constructs with OmpA protein fusions</h3>
-
<br>Paweł</h3>
+
<h4>Paweł</h4>
<p><ol>
<p><ol>

Revision as of 13:55, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Preparation of constructs with OmpA protein fusions

Paweł

  1. Clean-up of overnight digest.
  2. Ligation of OmpA_alfa and pET15b.
  3. Ligation of OmpA_omega and pET15b.
  4. Transformation of E. coli TOP10 strain with ligation products.
  5. Transformants plating on LB + ampicillin.