Team:Warsaw/Calendar-Main/4 July 2008
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- | <h3>Preparation of constructs with OmpA protein fusions< | + | <h3>Preparation of constructs with OmpA protein fusions</h3> |
+ | <h4>Paweł</h4> | ||
<p><ol> | <p><ol> | ||
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega). </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega). </li> | ||
- | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with SacI and BamHI (BamHI buffer). </li> |
<li> Gel electrophoresis. </li> | <li> Gel electrophoresis. </li> | ||
</ol></p> | </ol></p> |
Revision as of 14:03, 11 October 2008
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