Preparation of constructs with OmpA protein fusions

Michał K.

Clean-up of OmpA_alpha and OmpA_omega digest products.

Cloning PCL products on pKS

Michał L., Ewa, Marcin

1. Gel electophoresis of PCL products.
2. Gel-out of proper bands (alpha-A: 1500 bp and omega-A: 1440 bp).
3. Restriction digest of pKS vector and the PCL products with NotI and SacI (BamHI buffer) for 2 hours.
4. Ligation 1 hour.
5. Transformation of Top10 strain and screening on Amp₁₀₀ plates.