Team:Warsaw/Calendar-Main/11 July 2008

From 2008.igem.org

(Difference between revisions)
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<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
<p><ol>
<p><ol>
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<li> Colony PCR on colonies from plates with transformations pKS-A <br>
+
<li> Colony PCR on colonies from plates with transformations pKS-A. <br>
Primers used:
Primers used:
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
</li>
</li>
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin</li></ol></p>
+
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.</li></ol></p>

Revision as of 15:12, 11 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Colony PCR on colonies from plates with transformations OmpA_alpha.
  2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Colony PCR on colonies from plates with transformations pKS-A.
    Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

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