Brown: Team Resistance/9 July 2008

From 2008.igem.org

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*Initial OD 50mL culture: .038
*Initial OD 50mL culture: .038
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*                    .2%(1)  .2%(2)  .4%(1)  .4%(2)  .2% + Chloramphenicol(1)  .2% + Chloramphenicol(2)  Control
+
<nowiki>          .2%(1)  .2%(2)  .4%(1)  .4%(2)  .2% + Chloramphenicol(1)  .2% + Chloramphenicol(2)  Control
* 3:45 PM    .084    .085      .079  .079      .062                        .053                  .073
* 3:45 PM    .084    .085      .079  .079      .062                        .053                  .073
* 4:45 PM    .065    .069      .040  .048      .044                        .047                  .076
* 4:45 PM    .065    .069      .040  .048      .044                        .047                  .076
* 5:55 PM    .018    .018      .023  .021      .034                        .050                  .075
* 5:55 PM    .018    .018      .023  .021      .034                        .050                  .075
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* 6:50 PM    .014    .015      .013  .013      .050                        .044                  .071
+
* 6:50 PM    .014    .015      .013  .013      .050                        .044                  .071</nowiki>
*The first few readings indicated an increase in the optical density.  This is to be expected because the bacteria are in the mid-log phase and still growing.  Growth must occur in order for the S, R, and Rz genes to be expressed and translated.  Cell lysis is complete in four hours.
*The first few readings indicated an increase in the optical density.  This is to be expected because the bacteria are in the mid-log phase and still growing.  Growth must occur in order for the S, R, and Rz genes to be expressed and translated.  Cell lysis is complete in four hours.
*The addition of Chloramphenicol did not alter the rate of lysis.
*The addition of Chloramphenicol did not alter the rate of lysis.

Revision as of 16:15, 11 October 2008

  • OPTICAL DENSITY TEST:
  • We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
  • Initial OD 50mL culture: .038

.2%(1) .2%(2) .4%(1) .4%(2) .2% + Chloramphenicol(1) .2% + Chloramphenicol(2) Control * 3:45 PM .084 .085 .079 .079 .062 .053 .073 * 4:45 PM .065 .069 .040 .048 .044 .047 .076 * 5:55 PM .018 .018 .023 .021 .034 .050 .075 * 6:50 PM .014 .015 .013 .013 .050 .044 .071


  • The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated. Cell lysis is complete in four hours.
  • The addition of Chloramphenicol did not alter the rate of lysis.
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