Team:Warsaw/Calendar-Main/31 July 2008
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Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. | Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. | ||
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+ | <p>1. Isolation of plasmids from cultures inocluated on previous day.<br> | ||
+ | 2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains). | ||
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Revision as of 21:29, 11 October 2008
Checking if omp_omega_A_alpha gives ampicillin resistance
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