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Alberta NINT/30 May 2008
From 2008.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
lab work (WM): | lab work (WM): | ||
Miniprepped 8 O/Ns | Miniprepped 8 O/Ns | ||
| - | Digested | + | Digested K102010/(EcoRI + PstI) |
Sequence with VR primer | Sequence with VR primer | ||
| + | [[image: NINT_WMpg24b.JPG|500px]] | ||
Latest revision as of 22:12, 11 October 2008
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lab work (SD):
DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep
protocol.
This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
lab work (JD):
Counted colonies on ligation plates: negative plate:0, Positive plate:2
because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
lab work (WM):
Miniprepped 8 O/Ns
Digested K102010/(EcoRI + PstI)
Sequence with VR primer
