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Alberta NINT/31 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/30_July_2008 | < Previous entry]] | + | [[Alberta_NINT/30_July_2008 | < Previous entry]] | [[Alberta_NINT/1_August_2008 | Next entry >]] |
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lab work (JD): | lab work (JD): | ||
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properly | properly | ||
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | ||
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Latest revision as of 23:05, 11 October 2008
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lab work (JD):
Counted colonies on both mine and Shannon's plates from yesterday's ligations
-unfortunately equal numbers on negative plates and ligation plates so ligation
did not work
-my plate had 100s on the negative control so I assumed the vector was not cut
properly
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel
