Team:Warsaw/Calendar-Main/28 May 2008

From 2008.igem.org

(Difference between revisions)
Line 32: Line 32:
Digest was conducted with BamHI</var>
Digest was conducted with BamHI</var>
<div align=center>
<div align=center>
-
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/d/d6/Translation_fusion_digest_Eco.jpg"width=300/></a></div>
+
<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d6/Translation_fusion_digest_Eco.jpg"width=300/></a></div>
<var><b>Fig. 2.</b> 2 to 6 - Control digests of few clones with transcription fusion; <br>7 - pMPMT5-AID; all plasmids digested with EcoRI. </var>
<var><b>Fig. 2.</b> 2 to 6 - Control digests of few clones with transcription fusion; <br>7 - pMPMT5-AID; all plasmids digested with EcoRI. </var>

Revision as of 23:19, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcriptional fusion.
  2. Isolation of hypothetical pMPM-T5 with translational fusion.
  3. Control digest of pMPM-T5 with transcriptional fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (Fig. 1 and Fig. 2).
  6. Choice of proper clones and preparation for sequencing.

Rifampicin test

Michał L., Ewa, Marcin

  1. Inoculation of liquid LB with E.coli TOP10 carrying pMPM-T5+AID
  2. Inoculation of liquid LB with E.coli TOP10 carrying pTrc99A+AID


Fig. 1. Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
Digest was conducted with BamHI
Fig. 2. 2 to 6 - Control digests of few clones with transcription fusion;
7 - pMPMT5-AID; all plasmids digested with EcoRI.



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008"