Team:Warsaw/Calendar-Main/25 June 2008

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<li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (Fig.1.).</li></ol>
<li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (Fig.1.).</li></ol>
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<img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=300/>
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<img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/>
<var> <b>Fig. 1.</b> PCR product - gene coding A protein.</var>
<var> <b>Fig. 1.</b> PCR product - gene coding A protein.</var>

Revision as of 11:08, 12 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs and gel-out isolations from 16 June.

PCR of Alpha-link and link-A

Michał L. Marcin and Ewa

  1. We've ran gradient PCR to obtain Alpha-link and link-A.
    Annealing temp 48°C - 55°C.
    Elongation time 1 - 2 minutes.
  2. We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A (Fig.1.).
Fig. 1. PCR product - gene coding A protein.

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