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Team:Warsaw/Calendar-Main/27 June 2008
From 2008.igem.org
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<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
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| - | <li>Gel electophoresis of PCL products.</li> | + | <li>Gel electophoresis of PCL products (Fig. 1.).</li> |
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper bands (alpha-A: 1500 bp and omega-A: 1440 bp).</li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper bands (alpha-A: 1500 bp and omega-A: 1440 bp).</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Restriction digest</a> of pKS vector and the PCL products with NotI and SacI (BamHI buffer) for 2 hours.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Restriction digest</a> of pKS vector and the PCL products with NotI and SacI (BamHI buffer) for 2 hours.</li> | ||
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| + | <img src="https://static.igem.org/mediawiki/2008/e/ea/PCL_OmegaA_WAW.jpg" width=200/> | ||
| + | <var><b>Fig. 1.</b>PCL product - Omega-A fusion.</var> | ||
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Revision as of 11:16, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Clean-up of OmpA_alpha and OmpA_omega digest products. Cloning PCL products on pKSMichał L., Ewa, Marcin
Fig. 1.PCL product - Omega-A fusion.
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