"
Team:Warsaw/Calendar-Main/6 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 26: | Line 26: | ||
<h3> | <h3> | ||
| - | Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases | + | Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases (present in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a>).<br> |
Piotr</h3> | Piotr</h3> | ||
<p>Test was conducted in <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa. </p> | <p>Test was conducted in <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa. </p> | ||
| Line 36: | Line 36: | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Revision as of 13:20, 12 October 2008
 |
|
||||||||||||||||||||||||||||||||||||||||||||||||
 |
|
||||||||||||||||||||||||||||||||||||||||||||||||
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mM into same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100 mM) in ratio 1:50.
Conclusion: we obtained the best induction using 0.5 - 0.25 mM IPTG. Best ampicillin concentration is 50 - 75 μg/ml.
Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases (present in Top10).
| |||||||||||||||||||||||||||||||||||||||||||||||||
