Team:Warsaw/Calendar-Main/5 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
Line 37: | Line 37: | ||
<ol> | <ol> | ||
- | <li>Spinning</li> | + | <li>Spinning.</li> |
- | <li>Suspending</li> | + | <li>Suspending.</li> |
- | <li>Adding of lysis buffer</li> | + | <li>Adding of lysis buffer.</li> |
- | <li>Boiling</li> | + | <li>Boiling.</li> |
- | <li>Putting into poliacrylamide gel</li> | + | <li>Putting into poliacrylamide gel.</li> |
- | <li>Transfer onto nitrocellulose</li> | + | <li>Transfer onto nitrocellulose.</li> |
- | <li>Blocking</li> | + | <li>Blocking.</li> |
- | <li>Anti-A antibody binding</li> | + | <li>Anti-A antibody binding.</li> |
- | <li>Washing</li> | + | <li>Washing.</li> |
- | <li>Anti-rabbit antibody binding</li> | + | <li>Anti-rabbit antibody binding.</li> |
- | <li>Developing with BCIP and NBT</li> | + | <li>Developing with BCIP and NBT.</li> |
</ol> | </ol> | ||
Revision as of 13:36, 12 October 2008
Cloning of truncated fragment of protein APiotr
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation OmpA_omega_A_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary) Checking OmpA_omega_A_alpha and OmpA_A_alpha expressionPiotr
Preparing pACYC177+OmpA_omega_deltaA construct |