"
Team:Warsaw/Calendar-Main/11 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 14: | Line 14: | ||
Test was conducted in <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa. | Test was conducted in <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa. | ||
<ol> | <ol> | ||
| - | <li>Spinnign</li> | + | <li>Spinnign.</li> |
| - | <li>Suspending</li> | + | <li>Suspending.</li> |
| - | <li>Adding of lysis buffer</li> | + | <li>Adding of lysis buffer.</li> |
| - | <li>Boiling</li> | + | <li>Boiling.</li> |
| - | <li>Putting into poliacrylamide gel</li> | + | <li>Putting into poliacrylamide gel.</li> |
| - | <li>Transfer onto nitrocellulose</li> | + | <li>Transfer onto nitrocellulose.</li> |
| - | <li>Blocking</li> | + | <li>Blocking.</li> |
| - | <li>Anti-A antibody binding</li> | + | <li>Anti-A antibody binding.</li> |
| - | <li>Washing</li> | + | <li>Washing.</li> |
| - | <li>Anti-rabbit antibody binding</li> | + | <li>Anti-rabbit antibody binding.</li> |
| - | <li>Developing with BCIP and NBT</li> | + | <li>Developing with BCIP and NBT.</li> |
</ol> | </ol> | ||
</p> | </p> | ||
Revision as of 15:28, 12 October 2008
 |
|
||||||||
 |
|
||||||||
Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)PiotrTest was conducted in E.coli Rosetta strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa.
We didn't observe differences in espression and degradation in Rosettas nor in TOP10. Therefore we suppose that degradation of the fusions is caused by other factor than Lon and OmpT proteases.
|
|
