Team:Warsaw/Calendar-Main/23 August 2008

(Difference between revisions)

Revision as of 16:45, 12 October 2008


	Attribution analyzer / Interactive list of topics

Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI (Orange buffer), pET15b+OmpA_alpha dephosphorylation with CIAP
Gel electrophoresis of digested plasmids and gel-out of proper bands
Overnight ligation of pET15b+alfa and A

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 <h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3>
 <p><ol>
-<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008">friday's</a> ligation products. </li>
-<li> Transformants plating on LB + ampicillin.</li>
+<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI (Orange buffer), pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li>
+<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands  </li>
+<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of pET15b+alfa and A </li>
 </li>
 </ol></p>
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