From 2008.igem.org
(Difference between revisions)
|
|
| Line 8: |
Line 8: |
| | <p><ol> | | <p><ol> |
| | | | |
| - | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI (Orange buffer), pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li>
| |
| - | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li>
| |
| - | <li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of pET15b+alfa and A </li>
| |
| - | </li>
| |
| | | | |
| | + | </li> |
| | + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008">friday's</a> ligation products. </li> |
| | + | <li> Transformants plating on LB + ampicillin.</li> |
| | + | </li> |
| | | | |
| | </ol></p> | | </ol></p> |
Revision as of 16:45, 12 October 2008
 |
|
 |
|
Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni
- Transformation of E. coli TOP10 strain with friday's ligation products.
- Transformants plating on LB + ampicillin.
|
|
"
