Team:Warsaw/Calendar-Main/26 August 2008

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Revision as of 17:16, 12 October 2008


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Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
Lack of confirmed transformant colonies

Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha dephosphorylation with CIAP
Gel electrophoresis of digested plasmids and gel-out of proper bands
Ligation of pET15b+alfa and A
Transformation of E. coli TOP10.
Transformants plating on LB + ampicillin.

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 <html>
-<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
+<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
 <p><ol>
 <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pGeneart+A Primers used:
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 <li> Lack of confirmed transformant colonies </li></ol></p>
-<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid in place of OmpA<br>Antoni</h3>
+<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
 <p><ol>
 <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li>