Team:Warsaw/Calendar-Main/27 August 2008

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<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
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<p> Transformant colonies inoculated to liquid LB with ampicillin.</p>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin
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Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

Transformant colonies inoculated to liquid LB with ampicillin.

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