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Team:Warsaw/Calendar-Main/6 May 2008
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| - | <var><b>Fig. 1.</b> Probes of isolated genomic DNA after mechanical destruction. 1 and 3-<i>E.coli</i> Rosetta DNA; 2-DNA ladder; 4-<i>E.coli</i> TOP10 DNA.</var> | + | <var><b>Fig. 1.</b> Probes of isolated genomic DNA after mechanical destruction by pipetting. Without destruction the DNA wouldn't be visible in this kind of gel because genomic DNA is too big. 1 and 3-<i>E.coli</i> Rosetta DNA; 2-DNA ladder; 4-<i>E.coli</i> TOP10 DNA.</var> |
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Revision as of 20:13, 12 October 2008
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Preparation of pMPMT5+AID constructPiotr, Weronika
Isolation of genomic DNAMichał L.
Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control for PCR. Fig. 1. 
Fig. 1. Probes of isolated genomic DNA after mechanical destruction by pipetting. Without destruction the DNA wouldn't be visible in this kind of gel because genomic DNA is too big. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.
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